Department of Food Science and Biotechnology of National Chung Hsing University
國立中興大學 食品暨應用生物科技學系
黃菁英 助理教授
Ching Ying Huang, PhD., RD
生理學 博士
營養師
Assistant Professor
Lab of Gastrointestinal physiology, Mucosal immunology,& Nutrition
腸胃道生理與粘膜免疫實驗室
～延攬研究團隊～
腸道功能研究是21世紀最熱門的研究領域之一!
本實驗室成立於2017年夏天。歡迎本系大學部與研究所同學、醫學院營養相關科系或生命科學相關科系背景有志於腸道免疫與營養探索與研究之同學或專業人士加入本研究室團隊。
實際研究貢獻成果皆會列名發表於相關領域研討會與國際期刊。
E-mail: cyh@nchu.edu.tw (黃菁英 老師)
研究方向
以腸道功能為基礎之飲食/營養素研究
自古以來，腸道功能之恆定 (Homeostasis)，一直都受到東西方各派醫家之重視
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希臘醫學之父希波克拉底 (Hippocrates II, ~460 BC)
「一切疾病都是從腸道開始的」(All disease begins in the gut)
「讓食物成為你的藥物，你的藥物就是你的食物（Let food be thy medicine and medicine be thy food）。」
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中醫
黃帝內經(~2700 BC)「脾胃為後天之本」
金. 李東垣 脾胃論「內傷脾胃，百病由生」
以上皆呼應了當代關於腸道功能的研究，並顯示了其重要性。
腸道屏障功能
2007與2013年美國國家衛生研究院（National Institutes of Health, NIH）進行二階段「人體微生物組研究計畫（Human Microbiome Project）」，腸道生態系與腸道功能即成為當今科學界之熱門研究標的。
腸道屏障功能包含腸粘膜系統 (Intestinal mucosa), 主要由腸道上皮細胞 (intestinal epithelium)與固有層內的腸淋巴組織(gut-associated lymphoid tissue, GALT )之免疫細胞所組成，是人體消化道區隔外在物質與內在環境的第一屏障。
覆蓋於人體消化系統之上，是一單層上皮細胞，為區隔身體內在環境與消化管腔外在環境之介面。除了營養素的消化與吸收之外，在空間上，上皮細胞頂面(apical side)面對腸道微生物、食物分子、食品添加物、藥物...等外來物質; 底面 (basolateral side) 存在大量免疫細胞與神經叢，上皮肩負了雙向溝通之角色。位在腸組織腺窩(crypt)底部之腸幹細胞(intestinal stem cell)，規律地進行增生並分化成不同功能之腸上皮細胞，包括吸收型腸細胞 (absorptive enterocytes)、杯狀細胞 (goblet cells)、神經內分泌細胞 (neuroendocrine cells)、tuft cells、Paneth cells 與 M cells；而移動至頂端老舊細胞每3-5天發生凋亡。在新生與凋亡之間遞補更迭，不同功能之上皮細胞各司其職，形成巧妙之恆定。
You are what you EAT!
消化道之小腸是人體分解與攝取生命所需養份的關鍵器官，大腸則是將攝入養分去蕪存菁之後排出體外的中繼站。腸道上皮細胞、腸道管腔物質(微生物、食物分子、食品添加物、藥物)、腸免疫細胞之三相溝通與代謝反應，以及 Gut-organ axis (腸-器官軸)之溝通，構築與形塑人體各部機能之恆定。本實驗室之研究目標，以腸道生理學為基礎，探討單一或多重飲食因子 (dietary facctors) 對於腸道屏障功能之調節作用。
實驗室技術
大小鼠手術 (大鼠動靜脈氣管插管、腸道手術、心、肺、腎手術模式)、動物缺血模式、細胞株培養、肝臟初代細胞培養、腸道類器官培養、免疫染色技術、分生實驗、非致病性微生物培養... 等
About the Lab
Dr. Huang’s laboratory started up in the summer of 2017. Her research interests are grounded in intestinal physiology, aims to explore the regulatory effects of single or multiple dietary factors on intestinal barrier function. Utilizing the animal and cell culture model, she investigated the role of a single nutrient and mixed diet under physiological and pathological conditions. The techniques she used includes animal surgery, cell culture, intestinal organoid culture, non-pathogenic bacterial culture, immunoassay, histology stating and DNA & RNA extraction and analysis.
實驗室成員
碩士班 盧品錚 Pin-Cheng Lu
碩士班 黃琳蓉 Lin-Rong Huang
碩士班 洪子恬 Tzu-Tien Hung
碩士在職專班 楊閔喻 Min-Yu Yang
畢業生
碩士班 (2021.09-2023.08)蔡依璇 Yi-Syuan Tsai
大學部 (2021.07-2023-07) 吳靖堯 Ching-Yao Wu
碩士班 (2020.07-2023-02) 黃湘涵 Xiang-Han Huang
碩士在職專班 (2017.08-2022-02) 沈首如 Shou-Ju Shen
碩士班 (2019.07-2021-08) 郭亭攸 Ting-You Guo
碩士班 (2017.08-2019.08) 陳繼開 Ji-Kai Chen
Education
民國102年 國立台灣大學醫學院 生理所 博士
2013 National Taiwan University Physiology Ph.D.
民國93年 國立台灣大學醫學院 生理所 碩士
2004 National Taiwan University Physiology M.Sc.
民國91年臺北醫學大學 保健營養學系 學士
2002 Taipei Medical University Nutrition and Health Sciences B.Sc.
Experience
民國 106-迄今 國立中興大學食品暨應用生物科技學系 助理教授
2017-present National Chung Hsing University Department of Food Science and Biotechnology. Assistant Professor
民國 109-113 中國生理學會國際事務暨生理醫學教育委員會委員
2020-2024 International affair and physiology education committee (IAPEC) of the Chinese Physiological Society (CJP)
民國 105-106年 國立陽明大學生化暨分子生物研究所 博士後研究員
2016-2017 National Yang Ming University Biochem. & Mol. Biol. Post Doc.
民國 105年 銘傳大學生物醫學工程學系 兼任助理教授
2016 Ming-Chuan University Biomedical Engineering. Adjunct Assistant Professor
民國 105年 台北慈濟醫院影像醫學部 博士後研究員
2016 Department of Medical Imaging, Buddhist Tzu-Chi Hospital Post Doc.
民國 102-104年 國立台灣大學醫學院 生理所 博士後研究員
2013-2015 National Taiwan University Physiology Post Doc.
2006-2010 Elsevier Taiwan Project Consultant (Freelancer)
民國 93-95年 台大醫院外科部 研究助理
2004-2006 NTU Hospital Research assistant
民國 90-91年 台大醫院 營養師實習
2001-2002 NTU Hospital Dietetic Internships
Publications
Journal articles (期刊論文)
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Xiang-Han Huang(碩士班畢業生 黃湘涵), Ching-Ying Huang*.Fructose shields human colorectal cancer cells from hypoxia-induced necroptosis. npj science of food. 2024; Oct. DOI: 10.1038/s41538-024-00318-2
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Ting-You Guo (碩士班畢業生 郭亭攸), Wei-Ting Kuo, Yi-Syuan Tsai (碩士班畢業生 蔡依璇), Linda Chia-Hui Yu, Ching-Ying Huang*, Wei-Ting Kuo. Glucose-stimulated mucus secretion by goblet cells mitigates intestinal barrier dysfunction in a rat model of mesenteric ischemia/reperfusion injury. Current Developments in Nutrition. 2024;Jul 26. DOI:https://doi.org/10.1016/j.cdnut.2024.104431
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Ching-Ying Huang*, Ji-Kai Chen(碩士班畢業生 陳繼開), Wei-Ting Kuo. Glutamine induces remodeling of tight junctions in Caco-2 colorectal cancer cell. Medical Oncology .2022;Dec 2 (40):32. http://dx.doi.org/10.1007/s12032-022-01896-5.
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Deng-Fa Yang, Wen-Ching Huang, Changwei W. Wu, Ching-Ying Huang, Yu-Chen S. H. Yang, Yu-Tang Tung. Acute sleep deprivation exacerbates systemic inflammation and psychiatry disorders through gut microbiota dysbiosis and disruption of circadian rhythms. Microbiological Research. 2023;Dec (268):127292. http://dx.doi.org/10.1016/j.micres.2022.127292.
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Ya-Ting Wu, Shou-Ju Shen (在職班畢業生沈首如), Kuan-Fu Liao, Ching-Ying Huang*.Dietary Plant and Animal Protein Sources Oppositely Modulate Fecal Bilophila and Lachnoclostridium in Vegetarians and Omnivores. Microbiology Spectrum (2022) DOI:https://doi.org/10.1128/spectrum.02047-21.
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Huang CY, Yu LC. Distinct patterns of IL-12/23 and TNFα synthesis by activated macrophages is modulated by glucose and colon cancer metabolites. Chinese Journal of Physiology (2020);63(1):7.
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Huang CY, Huang CY, Pai YC, Lin BR, Lee TC, Liang PH, Chen CT, Yu LC. Glucose Metabolites Exert Opposing Roles in Tumor Chemoresistance. Frontiers Oncology 2019; 9: 1282. (doi: 10.3389/fonc.2019.01282)
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Huang CY, Kuo WT, Huang CY, Lee TC, Chen CT, Peng WH, Lu KS, Yang CY, and Yu LC (2017). Distinct cytoprotective roles of pyruvate and ATP by glucose metabolism on epithelial necroptosis and cryptproliferation in ischemic gut. Journal of Physiology doi: 10.1113/JP272208.
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Huang CY and Yu LC (2015) Pathophysiological mechanisms of death resistance in colorectal carcinoma World Journal of Gastroenterology 21(41):11777-11792 (review article)
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Wu LL, Peng WH, Kuo WT, Huang CY, Ni YH, Lu KS, Turner JR, and Yu LCH. (2014) Commensal bacterial endocytosis in epithelial cells is dependent on myosin light chain kinase-activated brush border fanning by interferon-gamma. American Journal of Pathology 184(8):2260-74.
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Huang CY, WT Kuo, Huang YC, Lee TC, Yu LC (2013) Resistance to hypoxia-induced necroptosis is conferred by glycolytic pyruvate scavenging of mitochondrial superoxide in colorectal cancer cells Cell Death Dis. 2013 May 2;4:e622. doi: 10.1038/cddis.2013.149. (over 110 citations)
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Lu YZ, Wu CC, Huang YC, Huang CY, Yang CY, Lee TC, Chen CF, Yu LC (2012) Neutrophil priming by hypoxic preconditioning protects against epithelial barrier damage and enteric bacterial translocation in intestinal ischemia/reperfusion.Lab Invest 95 (5):783-796. (over 60 citations)
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Huang CY, Hsiao JK, Lu YZ, Lee TC, Yu LC. (2011) Anti-apoptotic PI3K/Akt signaling by sodium/glucose transporter 1 reduces epithelial barrier damage and bacterial translocation in intestinal ischemia. Lab Invest. 91 (2):294-309. (over 90 citations)
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Hsiao JK, Huang CY, Lu YZ, Yang CY, and Yu LC (2009) Magnetic resonance imaging detects intestinal barrier dysfunction in a rat model of acute mesenteric ischemia/reperfusion injury. Invest Radiol.,44, 329-335.
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Yu LCH, Huang CY, WT Kuo, H Sayer, JR Turner, and AG Buret. (2008) SGLT-1-mediated glucose uptake protects human intestinal epithelial cells against Giardia duodenalis-induced apoptosis. International journal for parasitology, 38, 923-934. (over 70 citations)
International Conference abstracts (國際研討會)
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2024 Digestive Disease Week (Washington DC, USA) Oral presentation. Ching-Ying Huang ; Ting-You Guo; Sin-Rong Chen, Jing-Yi Wang. Epithelial-derived NADPH oxidase (NOX)-1 in enteral glucose-mediated restoration of crypt activity and reinforcement of barrier function in ischemic jejunum.Gastroenterology 2024;May(166):S-39.(conference abstract)
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2023 The 10th Federation of the Asian and Oceanian Physiological Societies Congress (Daegu, Korea) Ching-Ying Huang , Yi-Syuan Tsai, Jo-Chieh Liu .Oral administration of antioxidant modulated intestinal physiological balance
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2022 Digestive Disease Week (in person & virtual, San Diego, USA) Poster of distinction. Ching-Ying Huang , Ting-You Guo, and Xiang-Han Huang. Local enteral glucose restores crypt activity and fortifies barrier function on remote ischemic jejunum.Gastroenterology 2022;May (162):S-1050. http://dx.doi.org/10.1016/s0016-5085(22)62501-0. (conference abstract)
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2021 Digestive Disease Week (virtual) Poster of distinction. Ching-Ying Huang & Ting-You Guo. Local enteral glucose-stimulated spatial redistribution of mucus and goblet cells hyperplasia in remote intestinal segment ameliorated ischemia/reperfusion-induced mucosal inflammation.Gastroenterology 2021;May (160):S-237. http://dx.doi.org/10.1016/s0016-5085(21)01308-1.(conference abstract)
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2020 FASEB Experimental Biology Ching-Ying Huang & Ji-Kai Chen. Remodeling of tight junction protein zonula occludens (ZO)-1 by glutamine under glucose deprivation in colorectal cancer cells. The FASEB Journal, 34(S1), 1-1
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2018 Japan Digestive Disease Week (Kobe, Japan) Ching-Ying Huang and Linda C.H. Yu. Anti-inflammatory response of macrophage by epithelial cell-derived anaerobic lactate under hypoxic stress
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2016 Digestive Disease Week (San Diego, CA, USA)
Yen-Zhen Lu, Ching-Ying Huang, Tsung-Chun Lee, Wei-Ting Kuo, Yu- Chen Pai, Linda C.H. Yu. Immunomodulation by Hypoxic Preconditioning Prevents Mesenteric Ischemia/Reperfusion-Induced Bacterial Translocation in Rats: Role of TNFα-Dependent Neutrophil Priming. Gastroenterology, Vol.150, Issue 4, Page S494, 2016. -
2014 Digestive Disease Week (Chicago, IL, USA)
Li-Ling Wu, Wei-Hao Peng, Wei-Ting Kuo, Ching-Ying Huang, Yen-Hsuan Ni, Kuo-Shyan Lu, Jerrold R. Turner, Linda C.H. Yu. Gut Bacterial Endocytosis Is Dependent on MLCK-Activated Brush Border Fanning by IFN. Gastroenterology, Vol.146, Issue 5, Supplement 1, Page S-697, 2014,05. -
2013 FASEB Experimental Biology (Boston, MA, USA)
Ching-Ying Huang, Wei-Ting Kuo, Yi-Cheng Huang, Tsung-Chun Le2, and Linda C.H. Yu
Resistance to hypoxia-induced programmed necrosis is conferred by glycolytic pyruvate scavenging of mitochondrial superoxide in colorectal cancer cells -
2012 Digestive Disease Week (San Diego, CA, USA)
Ching-Ying Huang, Yen-Zhen Lu , Wei-Ting Kuo, Wei-Hao Peng, Kuo-Shyan Lu, Jong-Kai Hsiao, Chung-Yi Yang, and Linda C.H Yu. Cytoprotective mechanism by glycolytic ATP against receptor-interacting protein kinase-dependent programmed necrosis in ischemic enterocytes. Gastroenterology, Vol.142, Issue 5, Supplement 1, Page S-363, 2012,05 -
2010 Digestive Disease Week (New Orleans, LA, USA)
Ching-Ying Huang, Jong-Kai Hsiao, Yen-Zhen Lu, Linda C.H. Yu. Anti-Apoptotic PI3K/AKT Signaling Activated by Sodium/Glucose Transporter 1 Prevents Epithelial Barrier Damage and Bacterial Translocation in Intestinal Ischemic Rats. Gastroenterology, Vol.138, Issue 5, Supplement 1, Page S-608, 2010,05. -
2009 Digestive Disease Week (Chicago, IL, USA)
Ching-Ying Huang, Jong-Kai Hsiao, Yen-Zhen Lu, Linda C. Yu. Intestinal Epithelial SGLT1 Glucose Uptake Protects Against Mesenteric I/R Injuries of A3.poptosis-Dependent Barrier Defects and Bacterial Translocation in Rats. Gastroenterology, Vol. 136, Issue5, Supplement 1, Page A-773, 2009,05 -
2009 Digestive Disease Week (Chicago, IL, USA)
Li-Ling Wu, Ching-Ying Huang, Yen-Zhen Lu, Linda C. Yu. Epithelial MLCK and iNOS Are Involved in Mucosal Barrier Defects and BacterialTranslocation Induced By Ileal Obstruction. Gastroenterology, Vol. 136, Issue 5, Supplement 1, Page A-410, 2009,05. -
2008 FASEB Experimental Biology (San Diego, CA, USA)
Ching-Ying Huang, Jong-Kai Hsiao, Yen-Zhen Lu, Linda C.H. Yu. Luminal glucose protects against ischemia/reperfusion-induced intestinal epithelial barrier defects in rats. FASEB, 22:1120.11,2008,0
國內研討會 ( Since 2017)
第三十七屆生物醫學聯合學術年會 (2023-03)
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黃湘涵、黃菁英. Fructose protects against hypoxia-induced necroptosis in human colorectal cancer cells
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蔡依璇、黃菁英. Enteral antioxidant modulation of small intestinal reactive oxygen species production
第三十六屆生物醫學聯合學術年會 (2022-03)
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黃湘涵、郭亭攸、黃菁英. Investigation of the Mechanism Underling Local Addition of Luminal Glucose-Mediated Remote Mucus Secretion by Goblet Cell.
台灣家庭醫學醫學會 110年度學術研討會 (2021-10)
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吳雅婷、沈首如、黃菁英. Alteration of gut microbiota in the consumption of omnivores and vegetarian diet after colon cleansing
生理醫學研習會暨科技部研究成果發表會 (2020-08)
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郭亭攸、黃菁英. In situ administration of eEnteral glucose protects remote gut against gut ischemia-induced barrier defects dysfunction through remote inflammation suppression of intestinal inflammation.
第三十四屆生物醫學聯合學術年會 (2019-03)
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陳繼開、黃菁英. Glutamine-Mediated Tight Junction Remodeling under Metabolic Stress in Colorectal Cancer Cell.
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楊欣、黃菁英. Enteral glucose protects against gut ischemia-induced barrier defects through remote suppression of intestinal inflammation
第八屆海峽兩岸低氧及膳食健康學術研討會 (2017-09)
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余佳慧、黃菁英. Glucose-Mediated Cytoprotection in Gut Epithelium under Ischemic and Hypoxic Stress.
專利
專利名稱: 自動升降裝置及具有其自動升降裝置的跨細胞膜阻抗即時監測系統 (I797020, 中華民國, 2023)
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發明人: 蔣雅郁、黃菁英、徐漢翊、林承諺
Awards & Honers
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2024 Digestive Disease Week: Lecture oral presentation (Washington DC, USA, May 2024)
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2023 指導碩士班學生黃湘涵參加第三十八屆生物醫學聯合學術年會 獲得 永信李天德醫藥基金會壁報論文優等獎
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2022 Digestive Disease Week: Rated in the top 10% of all AGA abstracts selected for poster presentation (in Person & Virtual, San Diego, USA)
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2022 指導碩士班學生黃湘涵參加第三十七屆生物醫學聯合學術年會
獲得 永信李天德醫藥基金會壁報論文獎 (生理學會) -
2021 與台中慈濟醫院吳雅婷醫師合作參加台灣家醫年會海報獲得學會評選第三名 (碩士班學生沈首如參與研究，本人為通訊作者)
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2021 Digestive Disease Week: poster of distinction award (top 10% of all American Gastroenterological Association abstracts) (Virtual, USA)
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2020 指導碩士班學生郭亭攸參加 生理醫學研習會暨科技部研究成果發表會
獲得 優秀壁報論文 獎項 -
2018 Travel Award speaker, Japan Digestive Disease Week (Kobe, Japan)
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2017-2018科技部補助大專校院延攬特殊優秀人才
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2012 Digestive Disease Week: poster of distinction award (top 10% of all American Gastroenterological Association abstracts) (San Diego, CA, USA)
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2011 7th Congress of the Federation of Asian and Oceania Physiological Societies: Awardee in the graduate Student Competition (Taipei, Taiwan R.O.C.)
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2010 Digestive Disease Week: poster of distinction award (top 10% of all American Gastroenterological Association abstracts) (New Orleans, LA, USA)
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2009 Digestive Disease Week: poster of distinction award (top 10% of all American Gastroenterological Association abstracts) (Chicago, IL, USA)
Contact Me
中興大學食品暨應用生物科技學系206室
402台中市南區興大路145號
145 Xingda Rd., South Dist., Taichung City, Taiwan
Tel: 886-04- 22840385 ext.2020 (Office); 2061, 2062 (Lab)
E-mail: cyh@nchu.edu.tw
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